Salmonella detection in food products using M2 technology
Menon Biosensors, Inc. demonstrates its molecular diagnostic platform technology based on Nuclear Magnetic Resonance to detect Salmonella in seafood matrices.
San Diego, October 14, 2015 – Menon Biosensors, Inc. has developed a breakthrough assay that can detect Salmonella spp. in shrimp and fish tissues, without the need of sample enrichment in culture media. The experimental process to develop the assay was to spike Salmonella spp. genomic DNA into shrimp, fish and mussel matrices, DNA extraction and NMR detection using M2 technology.
Salmonella is one of the most common causes of food poisoning in the United States. According to the Center for Disease Control, Salmonella is estimated to cause one million illnesses in the United States, with 19,000 hospitalizations and 380 deaths each year. It is of big interest for the food and beverage industry to develop a specific and rapid detection system against this pathogen in order to reduce human health risks. Menon Biosensors has developed Salmonella spp. detection assays for several different seafood matrices (shrimp, fish, mussels) without the need for a prior bacterial enrichment step which typically requires at least 24 hours of sample incubation in an enriched medium for Salmonella growth. Menon system can perform 4 to 24 assays within1 to 2 hours, providing high-throughput, early detection of contaminated food.
Menon Biosensors has also demonstrated the specificity of the assay by spiking the same food matrices with a frequently occurring seafood bacterium, Vibrio parahaemolyticus. In this case, NMR did not generate any signal when using M2 technology with the Salmonella assay, indicating that the assay does not show cross-reactivity in the presence of other commonly-found seafood pathogens.
Menon’s Universal Sample Prep DNA extraction method has been optimized to a more user friendly and less time-consuming method, avoiding tedious steps with high risk of cross contamination.
The Menon team is currently working in the expansion of Salmonella assay to other food matrices (poultry, eggs, dairy products, etc.) together with the implementation of new target assays that represent a biohazard for consumers.
About Menon Biosensors, Inc.
Menon Biosensors is a molecular biochemistry company that provides DNA analysis for the diagnosis of biological pathogens. Menon Biosensors’ microfluidics and M2 NMR platform technology (classified by the U.S. Government until 2012) provides superior sensitivity and specificity and minimizes sample preparation, providing best in class sample-to-answer pathogen detection. The technology has been validated by respected third parties in more than 3,000 samples over six years.
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